General Cell Based Assays
Eurofins Discoverx PathHunter eXpress EGFR/ErbB2 Dimerization Assay, 2-Plate Kit. (93-1051E3CP14M)
The PathHunter EGFR/ErbB2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/ErbB2 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/ErbB2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioss KD-Validated Superoxide Dismut
Superoxide Dismutase 2 Primary Antibody Unconjugated Public Immunogen Range 251-314/428 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
Meso Scale Discovery U-PLEX Mouse IL-13 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-13 Assay Kit is a multiplex immunoassay that allows simultaneous measurement of mouse interleukin 13 (IL-13) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL compound calibrators controls 10X diluent read buffer wash buffer and adhesive plate seals The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL for mouse IL-13
Meso Scale Discovery U-PLEX Human TNF-B ASY
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures TNF-beta levels in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent tags calibrators and buffer The kit allows simultaneous measurement of TNF-beta and other biomarkers It can be used for biomarker discovery lead optimization and diagnostics
Molecular Devices Assay Kit, Fluorescent Calcium, Bulk, 96, 384, 1536-Well
The FLIPR Calcium 5 Assay Kit (Bulk) is a homogeneous, dye-based fluorescent assay designed for high-throughput measurement of intracellular calcium flux using FLIPR systems. The bulk configuration supplies reagents for large screening campaigns and supports 96-, 384-, and 1536-well plate formats.
- Homogeneous dye-based calcium flux assay for adherent and suspension cells.
- Bulk kit coverage: 100 plates for 96- or 384-well, 150 plates for 1536-well formats.
- Includes multiple vials of loading reagent; Component B (assay buffer) is not included in bulk kit.
- Amenable to incubation at 37°C or room temperature, enabling flexible workflows.
- Probenecid may be required for some cell lines; do not freeze loading buffer with probenecid.
- Store components at -20°C and 4°C; reagents are stable for six months in original packaging.
Cytek Biosciences Inc Cell Cycle Kit Amnis
Product Name: Muse Cell Cycle Kit
Cytek Catalog Number: MCH100106
Product Class: Guava Muse Reagent
Type: Muse
Size: 100 Tests
Eurofins Discoverx PathHunter Mouse MDC (CCR4) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse MDC (CCR4) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mCCR4 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ACROBiosystems Human Fc gamma RIIIA / CD16a (F176) binding Kit (TR-FRET) is expressed from Human, 10000 tests
Human Fc gamma RIIIA / CD16a (F176) binding Kit (TR-FRET) is expressed from Human
Eurofins Discoverx cAMP Hunter eXpress RXFP3 (SALPR) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0102E2CP2M)
The cAMP Hunter RXFP3 (SALPR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP3, a naturally Gi/Go coupled GPCR. Upon activation of RXFP3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences Matrix metalloproteinase-3 (MMP-3) fluorometric drug discovery kit, GREEN (1x96wells)
The MMP-3 Fluorometric (also known as fluorimetric)Drug Discovery Kit, GREEN is a complete assay system designed to screen MMP-3 inhibitors using a quenched fluorogenic MMP-3 substrate: 5-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(TQ2W)-NH2 [5-FAM=5-carboxyfluorescein; Nva=norvaline; TQ2W=quencher]. FAM fluorescence is thoroughly quenched by the TQ2W group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-3, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Stromelysin 1, Transin 1. Applications: Fluorescent detection, HTS Activity assay.
Enzo Life Sciences Matrix Metalloproteinase-14 (MMP-14) fluorometric drug discovery kit (1x96wells)
A QUANTIZYME Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OMNIMMP fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. Also included are active enzyme, a prototypic control inhibitor (NNGH3), and a detailed instruction booklet. Alternative name: Membrane type matrix metalloproteinase 1, MT-1 MMP. Applications: Fluorescent detection, HTS Activity assay.
Cayman Chemical NBD-Fructose, Purity: ≥95% (sum of isomers), CAS: 940961-04-6, 1 mg
A fluorescent derivative of fructose; has been used to monitor fructose uptake by the GLUT5 transporter in MCF-7, MDA-MB-435, and MDA-MB-231 human breast cancer cells; ex/em = 472/538 nm, respectively
Eurofins Discoverx PathHunter Human PPAR delta Nuclear Receptor Protein Interaction Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPAR delta Nuclear Receptor Stable Cell Line Protein Interaction Assay (CHO-K1) contains a stable clonal cell line used to measure activated PPARδ nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human ADRA2B β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human ADRA2B β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADRA2B (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.